|| Hearing loss due to genetic defects is one of the most frequent causes of hearing impairment. This sensory loss affects about 1 in 1,000 children in most parts of the world. In Egypt, hereditary non-syndromic hearing loss (NSHL) is more prevalent due to high rates of consanguineous marriages. Since connexin 26-associated hearing loss has been discovered in many parts of the world, we examined the prevalence of mutations in this gene in thirty-one Egyptian families with NSHL. Each family has between 2 to 5 cases of hearing loss, with no symptoms or signs suggestive of any other possible aetiology. Control subjects were studied to determine the carrier rate of connexin 26 in the general population (n=74). All samples were screened for the 35delg mutation using an allele-specific polymerase chain reaction (AS-PCR). We found the 35delg mutation is much less common in Egypt (about 10%) than in most other parts of the world, with a carrier rate of 2.7%.