NIH Mock Proposal
1. Specific Aims
2. Background and Significance
3. Research Design and Methods
   1. Yeast Genetics
   2. Immunopre- cipitation
   3. Yeast Two-Hybrid
   4. Future Experiments
4. References

Yeast YFH1 and human frataxin both localize to the mitochondria and both have been shown to be important in iron regulation. Mutants in both humans and yeast show a reduced level of Fe-S protein activity.⁹ In FRDA the pathological involvement of organs dependent upon effective mitochondrial function and metabolism further supports a mitochondrial function for frataxin.⁸ Additionally, a chimeric protein consisting of the N-terminal of YFH1 fused to residues 40-210 of human frataxin complements null yfh1 mutants.¹² This complementation provides very strong support for a homologous role of frataxin and YFH1. Yeast, therefore, are an excellent model system to identify the role of frataxin, especially since the S. cerevisiae genome has been sequenced.

3.1 Determination of protein interactions with YFH1 using yeast genetics

3.2 Determination of other protein interactions with frataxin through immunoprecipitation

3.3 Determination of protein interactions with frataxin through the yeast two-hybrid system

3.4 Future Experimentation

Next Section: 3.1 Determination of protein interactions with YFH1 using yeast genetics

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